To determine the oligomerization state of your protein of choice you only need to:

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Order your gene: Place the sequence of interest in the vector described in our publication.

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Express your protein: In our study we could easily express the protein in a standard Escherichia coli expression strain under native conditions.

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Purify the sample: Simply use a Ni affity chromatography to isolate your protein sample.

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Determine the fluorescence anisotropy: Record the fluorescence polarization of your sample with a plate reader (Tekan, PolarStar) or a spectrophotometer (Cary).

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Photobleach your sample: A simple setup to irradiate your sample is enough (laser diode pointing in a cuvette). The fluorescence intensity and anisotropy should be determined parallely.

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Use our benchmark values to calculate the oligomerization state of your protein!